Construction and identification of recombinant lentiviral vector containing HIV-1 Tat gene and its expression in 293T cells
نویسندگان
چکیده
منابع مشابه
Construction and identification of recombinant lentiviral vector containing HIV-1 Tat gene and its expression in 293T cells☆
OBJECTIVE To construct a lentiviral vector expressing HIV-1 Tat and identify its expression in 293T cells. METHODS The gene fragment of HIV-1 Tat101 was subcloned to lentiviral transfer vector pHAGE-CMV-MCS-IZsGreen, which was named pHAGE-Tat. Then the constructed pHAGE-Tat was used to co-transfect the packing 293T cells, together with the packaging plasmids pMD2.G and psPAX2. The packaged vi...
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ژورنال
عنوان ژورنال: Journal of Biomedical Research
سال: 2010
ISSN: 1674-8301
DOI: 10.1016/s1674-8301(10)60009-7